Composite
LCEC:FabV
Part:BBa_K2725004:Design
Designed by: Ben Astles Group: iGEM18_Edinburgh_UG (2018-10-11)
FabV Under Low Constitutive Expression Cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Various combinations of promoters and ribosome binding sites were tested before we settled on K314101.
Source
The fabV sequence was taken from the Vibrio fischeri genome, as shown on NCBI. K314101 was taken from the 2018 iGEM distribution kit.